Investigation on the Determination of Total Iron Binding Capacity of Serum Using Radioactive Iron 59Fe
نویسندگان
چکیده
منابع مشابه
Simplified determination of serum iron and total iron-binding capacity.
2,4,6-Tripyridyl-s-triazine (TPTZ) is a color reagent for ferrous ion in the pH range 2 to 10,when ascorbic acid is used as the reducing agent. In this range TPTZ and ferrous ion combine in the ratio of two to one. Tris buffer is commonly used to maintain an alkaline pH, but it reacts with other reducing agents, rendering them ineffective. These observations suggested a method for the joint det...
متن کاملDetermination of the Serum Iron-binding Capacity.
A colorimetric and an isotopic method for determining the iron-binding capacity are described, using only 1 ml. samples of plasma or serum. Both methods are simple, rapid and sensitive. Satisfactory results are obtained from frozen as well as from fresh samples.
متن کامل[Determination of serum iron and iron-binding capacity].
Rapid methods depending on differential absorptiometry are described for the determination of the transferrin iron content and the latent iron-binding capacity of blood serum. Each determination requires as little as 0.5 ml. serum. The methods are well adapted for routine use in the ;average' laboratory. Three or four sera may be completely analysed in 30 minutes. All operations are carried out...
متن کاملMethod for the determination of unsaturated iron-binding capacity of serum using radioactive iron and magnesium carbonate.
A method for measuring the unsaturated iron-binding capacity of serum using radioactive iron with magnesium carbonate as the adsorbing substance is described. The radioactive iron-magnesium carbonate method, compared with the methods of Caraway (1963), Herbert, Gottlieb, Lau, Fisher, Grevirtz, and Wasserman (1966), and Bothwell, Jacobs, and Kamener (1959), was shorter and simpler and equally re...
متن کاملSemi-automated micromethod for estimating the unsaturated iron-binding capacity of serum using radioactive iron.
A semi-automated micromethod for the determination of the unsaturated iron-binding capacity (UIBC) of serum using radioactive iron and magnesium carbonate as an adsorbing substance is presented. The method has the advantage of simplicity and speed. The determination of UIBC is carried out on 0.1 ml of serum in duplicate, using autodiluters, at the rate of up to 200 sera per day. The reproducibi...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1973
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.22.9_503